Improving the Resolution of the 2-Deoxy-D-Glucose Method,
نویسندگان
چکیده
منابع مشابه
Antiangiogenic Activity of 2-Deoxy-D-Glucose
BACKGROUND During tumor angiogenesis, endothelial cells (ECs) are engaged in a number of energy consuming biological processes, such as proliferation, migration, and capillary formation. Since glucose uptake and metabolism are increased to meet this energy need, the effects of the glycolytic inhibitor 2-deoxy-D-glucose (2-DG) on in vitro and in vivo angiogenesis were investigated. METHODOLOGY...
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A Novel LC-ELSD Method for the Quantification of 2-Deoxy D-Glucose Using HILIC Chromatography
A simple and novel method for the direct determination of 2-deoxy D-glucose (2DG) is developed, using a hydrophilic interaction chromatography (HILIC) and evaporative light scattering detection (ELSD). Altech Altima HP HILIC column (250 x 4.6 mm, 5μ particles) was used along with the mobile phase consisted of water and acetonitrile in a linear inverse gradient elution. The proposed method is ca...
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2-Deoxy-2-fluoro-d-glucose (FDG) is glucose analog routinely used in clinical and animal radiotracer studies to trace glucose uptake but it has rarely been used in plants. Previous studies analyzed FDG translocation and distribution pattern in plants and proposed that FDG could be used as a tracer for photoassimilates in plants. Elucidating FDG metabolism in plants is a crucial aspect for estab...
متن کاملAnticonvulsant and proconvulsant actions of 2-deoxy-D-glucose.
PURPOSE 2-Deoxy-D-glucose (2-DG), a glucose analog that accumulates in cells and interferes with carbohydrate metabolism by inhibiting glycolytic enzymes, has anticonvulsant actions. Recognizing that severe glucose deprivation can induce seizures, we sought to determine whether acute treatment with 2-DG can promote seizure susceptibility by assessing its effects on seizure threshold. For compar...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1981
ISSN: 0022-1554,1551-5044
DOI: 10.1177/29.1a_suppl.7288154